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For research use only.
| Test Principle | EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis. |
| Usage | This kit is for detection of IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17A, TNF-α and TNF-β in human serum, plasma, biological fluid and cell culture supernatant. |
| Product Type | Multi-Plex detection combination |
| Reacitivity | Human |
| Sample Type | serum,plasma,bodily fluid,cell culture supernatant |
| Sample Volume | 15 μL |
| Assay Time | 3 h |
| Detection Instrument | Flow cytometer |
| Fluor | PE |
| Ex/Em | 575/495,565 |
| Storage | The kit can be stored at 2～8℃ for up to 12 months by protecting the kit from light. |
| Expiration Ddate | 12 months |
Assay Procedures
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