Dual Luciferase Reporter Gene Luminescence Assay Kit (Glow Type) (E-BC-F074)
For research use only.
| Detection Principle |
Dual luciferase reporter gene is used to detect the luminescence reaction produced by luciferase catalyzed substrates. The detection system includes firefly luciferase reporter gene and renilla luciferase reporter gene. As an internal parameter of transfection, the renilla luciferase can normalize the experimental data. Firefly luciferase reporter gene refers to a reporting system that uses fluorescein as substrate to detect fluorescein luciferase activity. The reaction can occur in the presence of ATP, magnesium ions and oxygen, resulting in yellow-green fluorescence signals. The advantage of the reporter gene is that the expression product of the reporter gene has detectable enzyme activity as soon as the translation is completed. Renilla luciferase reporter gene refers to a reporting system that uses coelenterazine as substrate to detect the activity of renilla luciferase. This reaction does not depend on ATP to participate in the enzymatic reaction. During the reaction, a blue fluorescence signal is generated. The reaction principle of this kit is that in the presence of oxygen, ATP and magnesium ions at the same time, firefly luciferin and coelenterazine are catalyzed and oxidized successively by firefly luciferase and renilla luciferase in the samples to produce different fluorescence signals, and the expression levels of firefly luciferase and renilla luciferase in the samples can be detected by chemiluminescence immunoassay analyzer. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Chemiluminescence immunoassay analyzer or multifunctional microplate reader (with the function of detecting luminescence) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Assay Time | 40 min |
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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