DLK1 Polyclonal Antibody (E-AB-18225)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, 231 Verified Samples in IHC: Human colorectal cancer |
| Dilution | WB 1:500-1:2000, IHC 1:25-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human DLK1 |
| Abbre | DLK1 |
| Synonyms | Adipocyte differentiation inhibitor protein, Brevideltin, DLK, DLK 1, DLK-1, DLK1, Delta like 1 homolog, Delta like homolog, Delta like protein, Delta1, FA 1, FA1, Fetal antigen 1, Preadipocyte factor 1, Pref, Pref 1, Pref1, Protein delta homolog 1, Secredeltin, ZO, pG2 |
| Swissprot | |
| Calculated MW | 41 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane. |
| Concentration | 0.4 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Neuroscience, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a transmembrane protein containing six epidermal growth factor repeats.The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor.It is one of several imprinted genes located in a region of on chr 14q32.Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14).This gene is expressed from the paternal allele.A polymorphism within this gene has been associated with child and adolescent obesity. |
Other Clones
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Unconjugated
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