Dehydroascorbic Acid Colorimetric Assay Kit (E-BC-K864-M)
For research use only.
| Detection Principle |
Ascorbic acid (AsA) is an important physiological index of plant cells. The changes of its content, redox status (AsA/DHA ratio), and the activities of enzymes related to its synthesis and metabolism are involved in the response of plants to a series of environmental stresses. Dehydroascorbic acid (DHA) is a reversible oxidized form of AsA. In vivo, dehydroascorbic acid (DHA) and ascorbic acid form a redox system and act as an electron acceptor. The detection principle of this kit is that DTT reduces DHA to produce AsA, and the DHA content can be calculated by measuring the formation rate of AsA in the system at 265 nm. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (260-270 nm, optimum wavelength: 265 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.0016 μmol/mL |
| Detection Range | 0.03-1.00 μmol/mL |
| Precision | inter-assay CV: 2.5-4.2% | intra-assay CV: 3.5-5.6% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Photinia serrulata tissue homogenate | 2-4 |
| 10% Green pepper tissue homogenate | 1 |
| 10% Potato tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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