DDI2 Polyclonal Antibody (E-AB-18646)
For research use only.
| Verified Samples |
Verified Samples in WB: HL-60, Jurkat Verified Samples in IHC: Human tonsil |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human DDI2 |
| Abbre | DDI2 |
| Synonyms | DDI1 DNA damage inducible 1 homolog 2, DDI2, DNA damage inducible 1 homolog 2, DNA damage inducible 1 homolog 2 (S. cerevisiae), DNA damage inducible protein 2, Ddi2, MGC14844, Protein DDI1 homolog 2, RP4-680D5.5 |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1.32 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | DDI1 and DDI2 are ubiquitin receptor homologs of the Saccharomyces cerevisiae ddi1 protein, which is involved in regulation of the cell cycle and the late secretory pathway. DDI2 is a 399 amino acid protein that contains one ubiquitin-like domain and exists as three isoforms as a result of alternative splicing. The gene encoding DDI2 maps to human chromosome 1, the largest human chromosome which spans about 260 million base pairs and makes up 8% of the human genome. Other notable genes located on chromosome 1 include LMNA, which is associated with the rare aging disease Hutchinson-Gilford progeria, and the MUTYH gene, which is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome. |
Other Clones
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Unconjugated
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