DAO Polyclonal Antibody (AN006850L)
For research use only.
| Verified Samples | Verified Samples in WB: Rat kidney, Rat liver |
| Dilution | WB 1:1000-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Mouse Dao protein expressed by E.coli |
| Abbre | Dao |
| Synonyms | DAMOX, DAAO, Diamine Oxidase, D-amino-acid oxidase, Dao1, Dao |
| Swissprot | |
| Calculated MW | 39 kDa |
| Observed MW |
35-40 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, cytosol, Presynaptic active zone, Secreted |
| Tissue Specificity | Expressed in the kidney, cerebellum, pons, medulla oblongata and the cervical, thoracic and lumbar regions of the spinal cord. Expressed in goblet cells, enterocytes, mucosal contents and villous epithelium of the small intestine. In the epididymis, expression is higher in the caput region than in the corpus and cauda regions. In the thoracic and lumbar regions of the spinal cord, expression decreases in male mice with age. Expressed in neutrophils of the spleen following infection by S.typhimurium. Expression in the liver, heart and lung is disputed. In a model of ischemic stroke, expression is increased in cortical and hippocampal astrocytes following ischemia-reperfusion; an increase in expression and activity may lead to hydrogen peroxide-induced cytotoxicity. Expressed in the cerebellum and small intestine. Not expressed in the lung, cecum or cortex. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. |
Other Clones
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Unconjugated
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