CXCR4 Polyclonal Antibody (E-AB-31485)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse brain |
| Dilution | WB 1:500-1:2000, IF 1:100-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Fusin |
| Abbre | CXCR4 |
| Synonyms | C-X-C chemokine receptor type 4, CD184, CD184 antigen, CXC-R4, CXCR-4, CXCR4, Chemokine (C X C motif) receptor 4, Chemokine CXC Motif Receptor 4, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP 3, LAP3, LCR1, LESTR, Leuk, Leukocyte derived seven transmembrane domain receptor |
| Swissprot | |
| Calculated MW | 40 kDa |
| Observed MW |
36 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Metabolism, Immunology, Microbiology, Neuroscience, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1.The protein has 7 transmembrane regions and is located on the cell surface.It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells.Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome.Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
Other Clones
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Other Formats
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Unconjugated
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