CST3 Monoclonal Antibody (E-AB-22129)
For research use only.
| Verified Samples |
Verified Samples in WB: Recombinant protein Verified Samples in IHC: Human kidney Verified Samples in IF: Human liver cancer |
| Dilution | WB 1:1000-2000, IHC 1:100-200, IF 1:50-1:200 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, IHC-p, IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant Protein of Cystatin C of CST3 |
| Abbre | CST3 |
| Synonyms | AD 8, AD8, ARMD11, Amyloid angiopathy and cerebral hemorrhage, CST3 protein, CYTC, Cst 3, Cst3, Cystatin 3, Cystatin-3, Cystatin-C, Cystatin3, CystatinC, Epididymis secretory protein Li 2, Gamma trace, Gamma-trace, HCCAA, HEL S 2, MGC1173, bA218C14.4, bA218C14.4 (cystatin C) |
| Swissprot | |
| Observed MW |
14 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted. |
| Tissue Specificity | Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Cell Biology, Cardiovascular, Stem Cells, Tags and Cell Markers |
| Clone No. | 3B6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L. |
Other Clones
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Other Formats
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Unconjugated
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