COX4I1 Polyclonal Antibody (E-AB-70109)
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For research use only.
| Verified Samples |
Verified Samples in WB: Recombinant protein, Recombinant protein, Recombinant protein, Recombinant protein, Mouse heart, Mouse kidney, Rat heart, Rat kidney Verified Samples in IHC: Human tonsil, Human liver cancer, Mouse kidney cancer Verified Samples in IF: Rat brain |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:800, IF 1:200-1:500 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF |
| Clonality | Polyclonal |
| Immunogen | Recombinant protein corresponding to Mouse COX IV |
| Abbre | COX4I1 |
| Synonyms | AL024441, COX 4, COX IV, COX IV 1, COX IV-1, COX41, COX4B, COX4I1, COX4I2, COX4L2, COXIV, Cox4, Cox4a, Cytochrome C Oxidas, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, mitochondrial |
| Swissprot | |
| Calculated MW | 16 kDa |
| Observed MW |
16 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion inner membrane. |
| Concentration | 0.82 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Metabolism, Signal Transduction, Tags and Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | COX4I1,also named as COX4 and COXIV-1,belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase,the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2. |
Other Clones
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Unconjugated
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