CMPK1 Monoclonal Antibody (AN200144P)
For research use only.
| Verified Samples | Verified Samples in WB: K562 |
| Dilution | WB 1:500-1:2000, |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human CMPK1 Protein |
| Abbre | CMPK1 |
| Synonyms | CK, UMP-CMPK, CMK, CMPK, UCK, UMK, UMPK, CMPK1, CMPK 1, Cytidine monophosphate (UMP CMP) kinase 1 cytosolic, Cytidine monophosphate kinase, Cytidine monophosphate UMP CMP kinase 1 cytosolic, Cytidylate kinase, dCMP kinase, Deoxycytidylate kinase, EC 2.7.4.14, KCY, Monophosphatase kinase, OTTHUMP00000009565, RP11 511I2.1, UMP CMP, UMP CMP kinase, UMP/CMP kinase, UMP/CMPK, UMP-CMP kinase, Uridine monophosphate kinase, Uridine monophosphate/cytidine monophosphate kinase |
| Swissprot | |
| Calculated MW | 22 kDa |
| Observed MW |
22 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling, Metabolism |
| Clone No. | 13B7 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | CMPK1 plays a key role in the maintenance of pyrimidine nucleotide pool profile and for the metabolism of pyrimidine analogs in cells. It catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. CMPK1 also has a significant role in the activation of pyrimidine analogs, which are clinically useful anti-cancer and anti-viral drugs. In the meanwhile, CMPK1 functions in cellular nucleic acid biosynthesis. |
Other Clones
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Unconjugated
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