CK-7 Monoclonal Antibody (E-AB-70293)
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For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, A549, A431, Mouse lung, Mouse kidney, Mouse colon, Rat lung, Rat kidney, Rat colon Verified Samples in IHC: Human stomach cancer, Mouse stomach cancer, Rat stomach cancer |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:500 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal |
| Immunogen | Recombinant protein corresponding to Mouse Cytokeratin 7 |
| Abbre | CK-7 |
| Synonyms | 55K type II cytoskeletal, CK 7, CK-7, CK7, Cytokeratin 7, Cytokeratin-7, D15Wsu77e, K2C7, K7, Kerat, Keratin, Keratin 7, Keratin type II cytoskeletal 7, simple epithelial, simple epithelial type I, type II, type II cytoskeletal 7 |
| Swissprot | |
| Calculated MW | 51 kDa |
| Observed MW |
51 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 2.8 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Signal Transduction |
| Clone No. | 7D8G8 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells,which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins,including K9–K23,and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins,including K1–K8,and the hair keratins,Hb1–Hb6. KRT7,also named as cytokeratin 7,is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels,and their neoplasms. KRT7 is marker of epithelial tissues,but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7. |
Other Clones
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Other Formats
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Unconjugated
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