Citric Acid (CA) Colorimetric Assay Kit (E-BC-K351-S)
For research use only.
| Detection Principle | In acidic condition, Cr (Ⅵ) will be reduced to Cr3+, Cr3+ reacts with citric acid. And the product has a characteristic absorption peak at 545 nm, therefore the content of citric acid in sample can be calculated by measuring the absorbance value at 545 nm. |
| Synonyms | CA |
| Sample Type | Tissue,Mitochondria,Other liquid sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (545 nm) |
| Research Area | Mitochondria Function , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.05 mmol/L |
| Detection Range | 0.05-5.0 mmol/L |
| Precision | inter-assay CV: 5.4% | intra-assay CV: 4.2% |
| Sample Volume | 100 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 4-6 |
| 10% Mouse kidney tissue homogenate | 2-4 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 2-4 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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