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CG (Cholyglycine) ELISA Kit (E-EL-0183)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 18.75 ng/mL
Detection Range 31.25-2000 ng/mL
Sample Volume 100 μL
Manual Operation Time 1 h
Total Assay Time 3 h 30 min
Reacitivity Universal
Specificity This kit recognizes CG in samples.No significant cross-reactivity or interference between CG and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CG. During the reaction, CG in samples or Standard competes with a fixed amount of CG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CG. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase(HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of CG in the samples is then determined by comparing the OD of the samples to the standard curve.
Glycocholic acid (CG) is a conjugated bile acid formed by the combination of cholic acid and glycine, playing a crucial role in digestion by emulsifying fats. Its molecular structure features a steroid nucleus with hydroxyl groups linked to glycine.Synthesized in the liver, glycocholic acid is excreted into the bile to aid in fat digestion and absorption. It also regulates bile flow and cholesterol metabolism, protecting the liver from bile stasis and stone formation.Elevated serum glycocholic acid levels can indicate liver damage or dysfunction. Specific diseases associated with increased levels include acute and chronic hepatitis, cirrhosis, primary liver cancer, cholelithiasis, cholecystitis, and intrahepatic cholestasis of pregnancy (ICP). In ICP, glycocholic acid levels are significantly higher compared to healthy pregnant women, making it a useful diagnostic marker.Related factors such as serum total bile acids (TBA) and hyaluronic acid (HA) can provide additional information on liver function and fibrosis. Detection methods for glycocholic acid include radioimmunoassay (RIA) and latex immunoturbidimetry, with the latter being simpler and more cost-effective for routine clinical use.In summary, glycocholic acid is a valuable biomarker for assessing liver health and function.
Research Area Immunology , Metabolism
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