CFSE Cell Division Tracker Kit (E-CK-A345)
For research use only.
| Detection Principle | CFDA SE is a kind of fluorescein dye with membrane permeability, which has no fluorescence itself. When CFDA SE enters living cells through the cell membrane, it can be catalyzed by esterase in cytoplasm to produce carboxyfluorescein succinimide ester (CFSE), which can emit strong green fluorescence, can not penetrate the cell membrane, and can remain intact in the cell. CFSE can also spontaneously and irreversibly combine with amino groups of intracellular proteins to couple to these proteins. At the same time, excessive and uncoupled CFDA SE is passively diffused back into extracellular medium and removed by subsequent washing steps. |
| Detection Method | Fluorometric method |
| Sample Type | Cell samples |
| Detection Instrument | Flow Cytometer;Fluorescence Microscope |
| Dye Type | CFSE |
| Ex/Em | 494/521 |
| Channel Set | FITC |
| Other Reagents Required | PBS (pH7.2-7.6)(E-BC-R187). |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Expiration date | 12 months |
| Shipping | Ice bag |
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