CDX2 Monoclonal Antibody (E-AB-22020)
For research use only.
| Verified Samples |
Verified Samples in WB: 293T, Mouse heart Verified Samples in IHC: Human colon Verified Samples in IF: Mouse kidney |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p, IF |
| Clonality | Monoclonal |
| Immunogen | Synthetic Peptide |
| Abbre | CDX2 |
| Synonyms | CDX 2, CDX 3, CDX-3, CDX2, CDX2/AS, CDX3, Caudal type homeo box 2, Caudal type homeo box transcription factor 2, Caudal type homeobox 2, Caudal type homeobox protein 2, Caudal type homeobox transcription factor 2, Caudal-type homeobox protein 2, Cdx2, Homeobox protein |
| Swissprot | |
| Calculated MW | 34 kDa |
| Observed MW |
42 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Developmental Biology, Stem Cells |
| Clone No. | 1A5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene is a member of the caudal-related homeobox transcription factor gene family.The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation.This protein also plays a role in early embryonic development of the intestinal tract.Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. |
Other Clones
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Other Formats
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Unconjugated
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