CDC25A Polyclonal Antibody (E-AB-70140)
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For research use only.
| Verified Samples |
Verified Samples in WB: Hela, HepG2, Jurkat, Mouse spleen, Mouse stomach, Mouse colon, Rat spleen, Rat stomach, Rat colon Verified Samples in IHC: Human colon, Mouse breast cancer, Rat brain |
| Dilution | WB 1:500-1:1000, IHC 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Cdc25A |
| Abbre | CDC25A |
| Synonyms | CDC25A, CDC25A2, CDC25A2 CAG isoform, Cdc 25a, Cell division cycle 25 homolog A (S. pombe), Cell division cycle 25A, Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, D9Ertd393e, Dual specificity phosphatase Cdc25A, M phase inducer phosphatase 1 |
| Swissprot | |
| Calculated MW | 65-70 kDa |
| Observed MW |
70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. Nucleus. |
| Concentration | 0.96 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | CDC25A is a member of the CDC25 family of phosphatases.CDC25A is required for progression from G1 to the S phase of the cell cycle.It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups.CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division.CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.Two transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Other Formats
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Unconjugated
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