CDADC1 Polyclonal Antibody (E-AB-18595)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse heart Verified Samples in IHC: Human gastric cancer, Human lung cancer |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Full length fusion protein |
| Abbre | CDADC1 |
| Synonyms | CDAC1, CDADC1, Cytidine and dCMP deaminase domain-containing protein 1, MGC150615, MGC41774, MGC57136, NYD-SP15, OTTHUMP00000018405, RP11-103J18.2, Testis development protein NYD-SP, bA103J18.1, cytidine and dCMP deaminase domain containing 1, protein kinase NYD-SP15 |
| Swissprot | |
| Calculated MW | 58 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 0.6 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Metabolism, Signal transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | NYD-SP15 (testis development protein NYD-SP15), also known as CDADC1 (cytidine and dCMP deaminase domain containing 1), is a widely expressed protein with predominant expression in the testis, liver, spleen, kidney, thymus and placenta. NYD-SP15 is 514 amino acids in length and belongs to the cytidine and deoxycytidylate deaminase family. It is developmentally regulated with higher expression in adult testis than fetal testis and is believed to participate in spermatogenesis and testicular development. This suggests that NYD-SP15 may be a determining factor in male infertility. Due to alternative splicing events, four transcript variants exist for NYD-SP15. |
Other Clones
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Unconjugated
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