CD45/Ptprc Monoclonal Antibody (AN200170P)
For research use only.
| Verified Samples |
Verified Samples in WB:?Jurkat Verified Samples in IHC: Human spleen, Human lymphoma |
| Dilution | WB 1:500-1:2000, IHC-P 1:30-1:100 |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, IHC-P |
| Clonality | Monoclonal |
| Immunogen | A synthetic peptide corresponding to the C-terminus of the Human CD45/Ptprc |
| Abbre | PTPRC |
| Synonyms | B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, PTPRC, CD45 antigen, CD45R0, Ly-5, protein tyrosine phosphatase, receptor type, Receptor-type tyrosine-protein phosphatase C, CD45RO, Receptor-type tyrosine-protein phosphatase CLeukocyte common antigen (L-CA), T200, c polypeptide, CD 45, homolog of, Leukocyte common antigen, loc, Lyt-4, OTTHUMP00000033813, OTTHUMP00000033816, OTTHUMP00000033817, OTTHUMP00000038574, Protein tyrosine phosphatase receptor type c polypeptide, protein tyrosine phosphatase, receptor type, C, receptor type C, T200 glycoprotein, T200 leukocyte common antigen, B220, CD45, CD45 antigen, CD45R, GP180, L-CA, LCA, Ly-5, LY5, Protein tyrosine phosphatase, receptor type, Ptprc, Receptor-type tyrosine-protein phosphatase C, T200 |
| Swissprot | |
| Calculated MW | 143 kDa |
| Observed MW |
185 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Signal Transduction, Neuroscience, Stem Cells, Cardiovascular |
| Clone No. | 6C11 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin . Multiple splicing isoforms of exon 4 (A), 5 (B), and 6 (C) are expressed according to cell type, developmental stage and antigenic exposure . The longest form, CD45RABC (called B220 in mouse) is expressed on B lymphocytes, The shortest form, CD45R0, lacking exons 4, 5 and 6 which encode aa 34‑194, is expressed on memory cells, while intermediate sizes are expressed on other T cells. |
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Unconjugated
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