CD45 Polyclonal Antibody (E-AB-70378)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, RAW264.7, THP-1, Mouse thymus, Mouse lymph gland |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant protein corresponding to Mouse Human CD45. |
| Abbre | CD45 |
| Synonyms | B220, CD 45, CD45, CD45 antigen, CD45R, GP180, L-CA, LCA, LY5, Leukocyte common antigen, Ly-5, Ly5, Lyt-4, OTTHUMP00000033813, OTTHUMP00000033816, OTTHUMP00000033817, OTTHUMP00000038574, Protein tyros, Protein tyrosine phosphatase receptor type c polypeptide, homolog of, loc |
| Swissprot | |
| Calculated MW | 146 kDa |
| Observed MW |
180-240 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Membrane. |
| Concentration | 1.15 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Immunology, Neuroscience, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | CD45,also known as leukocyte common antigen (LCA),B220,and T200,is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells,except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth,differentiation,cell cycle,and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29,CD44,CD90,CD105 and CD166),negative selection markers (such as CD34 and CD45) are used for MSC identification. |
Other Clones
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Other Formats
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Unconjugated
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