CD44 Polyclonal Antibody (D-AB-10184L)
For research use only.
| Verified Samples |
Verified Samples in WB: Human A549, Human Hela, Mouse RAW264.7, Mouse spleen, Rat spleen |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Rat CD44 protein expressed by E.coli |
| Abbre | CD44 |
| Synonyms | BA-1, CD44 antigen, CD44 molecule, CSPG8, Cell surface glycoprotein CD44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor , HAS, HCELL, HUTCH1, PGP-1, Phagocytic glycoprotein 1, chondroitin sulfate proteoglycan 8, LHR |
| Swissprot | |
| Calculated MW | 51 kDa |
| Observed MW |
70-85 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Immunology, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration,tumor growth and progression. CD44 is also involved in lymphocyte activation,recirculation and homing,and in hematopoiesis. This protein exists in multiple isoforms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and undergoes extensive post-translational modifications. |
Other Clones
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Unconjugated
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