Cathepsin D (CatD) Activity Fluorometric Assay Kit (E-BC-F052)
For research use only.
| Detection Principle |
Cathepsin D (CatD) is an important intracellular aspartic acid protease and is widely present in the biological world. Cathepsin D has multiple biological functions and is involved in intracellular protein degradation, apoptosis and autophagy, etc. The detection principle of this kit: CatD hydrolyzes the substrate to generate the chromogenic substance. The activity of cathepsin D in the sample is calculated by measuring the changes in fluorescence values at the excitation wavelength of 328 nm and the emission wavelength of 393 nm |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=328 nm/393 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | PBS (0.01 M, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.02 U/L |
| Detection Range | 0.02-30.00 U/L |
| Precision | inter-assay CV: 3.6-4.7% | intra-assay CV: 2.3-3.1% |
| Sample Volume | 20 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Mouse serum | 1-2 |
| Mouse plasma | 1 |
| 10% Rat pancreas tissue homogenate | 1-2 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat small intestine tissue homogenate | 1-2 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat stomach tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 2-4 |
| 10% Rat liver tissue homogenate | 2-8 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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