Catenin beta Polyclonal Antibody (E-AB-70005)
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For research use only.
| Verified Samples |
Verified Samples in WB: HCT116, Mouse lung, Mouse brain, Rat brain Verified Samples in IHC: Human colon, Mouse kidney, Human colon Verified Samples in IF: Mouse stomach |
| Dilution | WB 1:500-1:2000, IHC 1:300-1:1000, IF 1:200-1:800 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse β-catenin |
| Abbre | Catenin beta |
| Synonyms | 88kDa, Beta catenin, Beta-catenin, CATNB, CHBCAT, CTNB1, CTNNB, CTNNB1, Cadherin associated protein, Catenin (cadherin associated protein), Catenin beta 1, Catenin beta-1, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000162082, OTTHUMP0000016522, OTTHUMP00000165222, beta 1 |
| Swissprot | |
| Calculated MW | 92 kDa |
| Observed MW |
92 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm>cytoskeleton. Cell junction>adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. |
| Concentration | 0.97 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Neuroscience, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | CTNNB1,also known as β-Catenin,is an evolutionarily conserved,multifunctional intracellular protein. CTNNB1 was originally identified in cell adherens junctions (Ajs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the Ajs,CTNNB1 is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. Deregulation of CTNNB1 activity is associated with multiple diseases including cancers. . |
Other Clones
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Unconjugated
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