Caspase 2 Activity Assay Kit(Colorimetric Method) (E-CK-A382)
For research use only.
| Detection Principle | Caspase 2, also known as Ich-1(ICE and CED-3 homolog 1) or Nedd-2, can be activated during signal transduction of apoptosis. Caspase 2 mRNA can be cleaved into two different forms. The mRNA product of full-length caspase 2 can be promote apoptosis, while the protein product of short mRNA can inhibit apoptosis. Caspase 2 can be activated by caspase 1, caspase 3 and granzyme B in vitro. This caspase 2 activity assay kit is based on caspase 2 that can catalyze the substrate Ac-VDQQD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 2 can be calculated by determining the absorbance at 405 nm. |
| Detection Method | Colorimetric method |
| Sample Type | Cell and tissue samples |
| Assay Time | 4-6 hours |
| Detection Instrument | Spectrophotometer |
| Other Reagents Required | PBS(E-BC-R187), Bradford Protein Determination Kit(E-BC-K168-S or E-BC-K168-M) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Expiration date | 6 months |
| Shipping | Ice bag |
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