Carboxylesterase (CES) Activity Colorimetric Assay Kit (E-BC-K579-M)
For research use only.
| Detection Principle |
Carboxylesterase (CES) is a polymeric protein that can catalyze the hydrolysis of carboxylate, thioester, carbamate and amide esters. CES can release effective drugs from some insoluble carboxylate prodrugs through hydrolysis reaction according to its hydrolysis characteristics. Secondly, due to its high catalytic efficiency and high specificity, CES can also regulate various metabolic functions (metabolism of ester substances, gene expression, material transport and detoxification, etc.). Therefore, detecting the activity of CES is of great significance for the early diagnosis of cancer and the research of biomedicine. CES catalyzed substrate reaction to produce chromogenic substance has maximum absorption at wavelength 405 nm, and CES enzyme activity is calculated by measuring OD value at 405 nm. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-410 nm, optimum wavelength: 405 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.3 U/L |
| Detection Range | 0.3-168.4 U/L |
| Precision | inter-assay CV: 2.7-11.0% | intra-assay CV: 1.8-5.1% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 100-300 |
| 10% Mouse kidney tissue homogenization | 100-300 |
| 10% Rat liver tissue homogenization | 100-300 |
| 10% Mouse brain tissue homogenization | 10-20 |
| 10% Mouse heart tissue homogenization | 10-20 |
| Rats serum | 10-20 |
| Rats plasma | 10-20 |
| Human serum | 10-20 |
| Mouse serum | 10-20 |
| Mouse plasma | 10-20 |
| 1×10^6 293T cells | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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