Ca2+-ATPase Activity Assay Kit (E-BC-K212-M)

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Detection Principle	ATPase can decompose ATP to produce inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. In the control system, Ca²⁺ -ATPase activity was inhibited, while in the sample system, Ca²⁺ -ATPase activity was not inhibited. The difference of inorganic phosphorus content between the sample and the control was the inorganic phosphorus produced by Ca²⁺ -ATPase during the incubation time. The activity of Ca²⁺ -ATPase was determined by inorganic phosphorus production.
Synonyms	Ca2+-ATPase
Sample Type	Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (660 nm)
Research Area	Oxidative Stress
Other Reagents Required	Ultrapure water, Normal saline (0.9% NaCl)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.18 U/kg wet weight
Detection Range	1.18-286.43 U/kg wet weight
Precision	inter-assay CV: 0.08 \| intra-assay CV: 0.06
Sample Volume	400 μL(tissue homogenate)
Assay Time	1 h 30 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat liver tissue homogenate	5-8
10% Mouse liver tissue homogenate	1
10% Rat heart tissue homogenate	5-8
10% Rat lung tissue homogenate	5-8
10% Rat kidney tissue homogenate	5-8
10% Rat brain tissue homogenate	2-3

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.