Blood Ammonia Colorimetric Assay Kit (E-BC-K145-M)

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	96T	$ 260.00
	48T	$ 180.00

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Detection Principle	Blood protein can be precipitated with protein precipitator, and enzyme activity will be destroyed, which can prevent the formation of free ammonia in vitro. Most interfering color substances were removed at the same time, indigo was formed in non-protein filtrate by Berthelot reaction, and the color depth was proportional to the content of blood ammonia. Blood ammonia content can be determined by comparing with standard solution.
Sample Type	Serum,Plasma
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (600-660 nm, optimum wavelength: 635 nm)
Research Area	Liver And Renal Function , Amino Acids And Proteins
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.01 mmol/L
Detection Range	0.01-2.5 mmol/L
Precision	inter-assay CV: 7.2% \| intra-assay CV: 4.1%
Sample Volume	0.1 mL
Assay Time	45 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Human plasma	1
Mouse serum	1
Rat plasma	1
Dog serum	1
Horse serum	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.