beta Tubulin Monoclonal Antibody (E-AB-20033)
For research use only.
| Verified Samples |
Verified Samples in WB: A549, Rat brain, Mouse brain, Chicken lung, Rabbit testis, Sheep muscle Verified Samples in IHC: Mouse testis Verified Samples in IF: Hela |
| Dilution | WB 1:5000-1:10000, IHC 1:100-1:300, IF 1:100-1:300 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat, Monkey, Chicken, Dog, Hamster, Rabbit, Sheep, Insect, Yeast |
| Applications | WB, IHC-p, IF |
| Clonality | Monoclonal |
| Immunogen | Synthetic Peptide |
| Abbre | β-Tubulin |
| Synonyms | CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, M(beta)3, M(beta)6, MC1R, Neuron specific beta III Tubulin, Neuron-specific class III beta-tubulin, QccE-11995, QccE-15186, TBB3, TUBB3, TUBB4, Tubb 3, Tubulin, Tubulin beta 3, Tubulin beta 3 chain, Tubulin beta 4, beta 3 tubulin, beta-4 |
| Swissprot | |
| Calculated MW | 50 kDa |
| Observed MW |
55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm>cytoskeleton. |
| Tissue Specificity | Expression is primarily restricted to central and peripheral nervous system. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Neuroscience, Signal Transduction, Tags and Cell Markers |
| Clone No. | 8B2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. |
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Other Formats
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Unconjugated
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