BCAS3 Polyclonal Antibody (E-AB-18703)
For research use only.
| Verified Samples |
Verified Samples in WB: 293T Verified Samples in IHC: Human lung cancer, Human colorectal cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human BCAS3 |
| Abbre | BCAS3 |
| Synonyms | 1500019F07Rik, 2610028P08Rik, AU021018, BC028339, BCAS3, Breast carcinoma-amplified sequence 3, FLJ20128, GAOB1, K20D4, MAAB, MGC4973, Maab1 protein, rudhira |
| Swissprot | |
| Calculated MW | 101 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. |
| Concentration | 1.26 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | BCAS3 (breast carcinoma amplified sequence 3), also designated MAAB or GAOB1, is a 913 amino acid protein that is believed to be involved in breast cancer progression.The gene is regulated by ER (estrogen receptor alpha) and expressed in multiple tissues, including malignant human brain lesions.It is overexpressed and amplified in breast cancer cell lines.BCAS3 contains three WD40 repeat regions, a bromodomain, a rare zinc-finger motif, four probable DNA-binding domains, and two kinase-inducible phosphorylation domains.Five variants are produced due to alternative splicing.BCAS3 interacts with histone H3 and PCAF, which is indicative of histone acetyltransferase activity.BCAS3 also exhibits ER transactiviation activity by acting as a coactivator with PELP1 or MTA1.The amplification and translocation between the BCAS3 gene and the BCAS4 gene results in a fusion transcript is overexpressed in MCF-7 cells. |
Other Clones
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Unconjugated
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