ATP Chemiluminescence Assay Kit (Double Reagent) (E-BC-F300)
For research use only.
| Detection Principle | Luciferase combines with luciferin and undergoes an oxidation reaction in the presence of ATP and oxygen, releasing fluorescence. When both luciferase and luciferin are in excess, within a certain range, the intensity of the light signal is proportional to the ATP content. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Chemiluminescence immunoassay analyzer or multifunctional microplate reader |
| Research Area | Oxidative Stress , Tricarboxylic Acid（TCA）Cycle , Mitochondria Function , Energy Metabolism |
| Other Reagents Required | PBS(0.01 M，pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.002 μmol/L |
| Detection Range | 0.002-1 μmol/L |
| Precision | inter-assay CV: 3.4-5.2% | intra-assay CV: 1.7-2.6% |
| Sample Volume | 100 μL(cell homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1×10^4 Jurkat cells | 1 |
| 1×10^4 RAW 264.7 cells | 1 |
| 1×10^4 HL-60 cells | 1 |
| 1×10^4 Hela cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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