ATG16L1 Polyclonal Antibody (E-AB-53360)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela, Raji Verified Samples in IHC: Human breast cancer, Human thyroid cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human ATG16L1 |
| Abbre | ATG16L1 |
| Synonyms | A16L1, APG16 like 1, APG16-like 1, APG16L, APG16L beta, ATG16 autophagy related 16 like 1, ATG16 autophagy related 16-like 1 (S. cerevisiae), ATG16A, ATG16L, Atg16l1, Autophagy related protein 16 1, Autophagy-related protein 16-1, FLJ00045, FLJ10035, FLJ10828, FLJ22677 |
| Swissprot | |
| Calculated MW | 68 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. |
| Concentration | 0.8 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Cell Biology, Cardiovascular, Metabolism, Neuroscience, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | ATG16L1 (Autophagy Related 16 Like 1) is a Protein Coding gene. Diseases associated with ATG16L1 include Inflammatory Bowel Disease 10 and Inflammatory Bowel Disease. Among its related pathways are Autophagy Pathway and Senescence and Autophagy in Cancer. GO annotations related to this gene include identical protein binding. An important paralog of this gene is ATG16L2.The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Unconjugated
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