AST Polyclonal Antibody (D-AB-10389L)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela, HEK-293T, HepG2, NIH-3T3, C2C12, Mouse liver, Mouse brain, Rat liver, Rat brain |
| Dilution | WB 1:500-1:1000 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Mouse AST protein expressed by E.coli |
| Abbre | AST |
| Synonyms | Aspartate aminotransferase, Fatty acid-binding protein (FABP-1), Glutamate oxaloacetate transaminase 2 Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transam, Kynurenine--oxoglutarate transaminase 4, mAspAT, mitochondrial |
| Swissprot | |
| Calculated MW | 47 kDa |
| Observed MW |
41 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle,it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol,and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids. |
Other Clones
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Other Formats
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Unconjugated
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