Asparaginase (ASNase) Activity Colorimetric Assay Kit (E-BC-K909-M)
For research use only.
| Detection Principle |
Asparaginase (ASNase) is a proteolytic enzyme that was first discovered in guinea pigs and is not naturally produced in humans. Asparaginase has been widely used in the food industry, inhibiting the production of acrylamide carcinogens by hydrolyzing asparagine. Asparaginase is also used to treat various diseases due to its unique anti-tumor properties, such as acute lymphocytic leukemia, non-Hodgkin's lymphoma, leukemia meningitis, lung cancer and ovarian cancer and other tumor diseases. The detection principle of this kit is as follows: asparaginase catalyzes the substrate to generate aspartic acid, and aspartic acid is hydrolyzed under the catalytic action of the enzyme. The amount of NADH consumed by the reaction is directly proportional to the activity of asparaginase. NADH is absorbed at a wavelength of 340 nm, and the activity of asparaginase can be calculated based on the change in absorbance value per unit time. |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | PBS(0.01 M，pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.27 U/L |
| Detection Range | 1.27-182.52 U/L |
| Precision | inter-assay CV: 7.1-8.2% | intra-assay CV: 2.2-4.7% |
| Sample Volume | 40 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Guinea pig serum | 2-15 |
| Guinea pig plasma | 2-15 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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