Arginase Activity Colorimetric Assay Kit (E-BC-K848-M)

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	96T	$ 450.00

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Detection Principle	Arginase is a key enzyme in the urea cycle. Arginase plays an important role in tumor, cardiovascular disease and so on. The principle of this kit is that arginase catalyzes substrate decomposition to produce urea, and urea reacts with chromogenic agent to produce chromogenic substance. The optimal detection wavelength of this product is 520 nm. By detecting the amount of chromogenic substance produced per unit time, the activity of arginase in the sample can be calculated.
Sample Type	Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (500-540 nm, optimum wavelength: 520 nm)
Research Area	Oxidative Stress , Amino Acids And Proteins
Other Reagents Required	Normal saline (0.9% NaCl), Deionized water
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.07 U/L
Detection Range	0.07-23.40 U/L
Precision	inter-assay CV: 7.2-10.0% \| intra-assay CV: 2.5-4.8%
Sample Volume	50 μL(tissue/cell homogenate)
Assay Time	1 h

Cat.No.	Product Name	Sizes
E-BC-K016-M	Uric Acid (UA) Colorimetric Assay Kit	96T , 48T
E-BC-K850-M	L-Arginine (L-Arg) Colorimetric Assay Kit	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse kidney tissue homogenate	1
10% Mouse brain tissue homogenate	1
10% Mouse heart tissue homogenate	1
10% Mouse liver tissue homogenate	20-120
1×10^6 293T cells	1
1×10^6 HepG2 cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.