ANXA5 Polyclonal Antibody (E-AB-40386)
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For research use only.
| Verified Samples |
Verified Samples in WB: Hela, A431, HepG2, NIH/3T3, Rat lung, Mouse lung Verified Samples in IHC: Human placenta, Mouse lung, Rat lung |
| Dilution | WB 1:1000-1:3000, IHC 1:100-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human Annexin A5 protein |
| Abbre | ANXA5 |
| Synonyms | ANX A5, ANX5, ANXA5, Anchorin CII, Annexin 5, Annexin A5, Annexin V, Annexin-5, CBP-I, Calphobindin I, ENX2, Endonexin II, Lipocortin V, PAP I, PAP-I, PLACENTAL PROTEIN 4, Placental anticoagulant protein 4, Placental anticoagulant protein I, Pp4, Thromboplastin inhibi |
| Swissprot | |
| Calculated MW | 35 kDa |
| Observed MW |
35 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Secreted, Membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cell Biology, Cancer, Cardiovascular |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Annexin A5 (ANXA5),is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction,inflammation,growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I,vascular anticoagulant-alpha,endonexin II,lipocortin V,placental protein 4 and anchorin CII. |
Other Clones
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Other Formats
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Unconjugated
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