Annexin V-PE Reagent (E-CK-A115)

All	Size	Price	Qty
	500Tests	$ 660.00
	200Tests	$ 298.00
	100Tests	$ 190.00
	50Tests	$ 110.00

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Detection Principle	Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope.
Detection Method	Fluorometric method, Flow cytometry
Sample Type	Cell samples
Assay Time	40 min
Detection Instrument	Flow Cytometer
Dye Type	PE
Ex/Em	495;565/575
Channel Set	PE
Other Reagents Required	Annexin V Binding Buffer(E-CK-A151), PBS(E-BC-R187), Deionized water
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Expiration date	12 months
Shipping	Ice bag

Cat.No.	Product Name	Sizes
E-CK-A151	Annexin V Binding Buffer (10×)	50mL , 10mL
E-CK-A216	Annexin V-PE/7-AAD Apoptosis Kit	200Assays , 100Assays , 50Assays , 20Assays
E-CK-A256	Annexin V-PE/DAPI Apoptosis Kit	200Assays , 100Assays , 50Assays , 20Assays
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)	100Assays , 50Assays , 20Assays
E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)	100Assays , 50Assays , 20Assays

1 Results

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1 Results

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Q1:Will using EDTA-containing trypsin affect Annexin V staining?
EDTA-containing trypsin can be used, but ensure thorough washing with PBS after digestion to remove residual EDTA.
Q2:Can Annexin V kit be detected using fluorescence microscopy?
Yes, Annexin V kit can be used with fluorescence microscopy to observe apoptosis in situ. However, apoptotic cells often exhibit reduced adhesion (e.g., poor attachment or floating), which may result in detection failure during observation.