Annexin A1 Polyclonal Antibody (AN007180L)
For research use only.
| Verified Samples | Verified Samples in WB: A431, C2C12, C6 |
| Dilution | WB 1:5000-1:10000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human Annexin A1 protein expressed by E.coli |
| Abbre | Annexin A1 |
| Synonyms | ANX, Annexin I (lipocortin I), AnnexinA1, AnnexinI, ANX 1, ANX A1, ANXA 1, ANXA1 protein, CalpactinII, Chromobindin9, HGNC:533, Lipocortin 1, lipocortin I, ANX1, LPC1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35, Lipocortin1, LipocortinI, LPC 1, LPC, ANXA, Annexin A, ANXA1, ANX1, LPC1, Annexin 1, Calpactin 2, Chromobindin 9, Annexin-1, Chromobindin-9, p35, Calpactin-2, Annexin A1, Annexin I, Calpactin II, Lipocortin I, Phospholipase A2 Inhibitory Protein |
| Swissprot | |
| Calculated MW | 38 kDa |
| Observed MW |
35 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Cytoplasm, Cell projection, Cilium, Basolateral cell membrane, Found in the cilium |
| Tissue Specificity | Detected in resting neutrophils,peripheral blood T-cells,extracellular vesicles in blood serum from patients with inflammatory bowel disease. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells.Has no effect on unstimulated T cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton.Has high affinity for Ca2+ and can bind up to eight Ca2+ ions. Displays Ca2+-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca2+-dependent interaction between phagosomes and the actin cytoskeleton. |
Other Clones
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Unconjugated
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