Angiotensin I Converting Enzyme 2（ACE2）Activity Fluorescence Assay Kit (E-BC-F054)
For research use only.
| Detection Principle |
Angiotensin converting enzyme 2 (ACE2) is an important component of the renin angiotensin system (RAS). ACE2 is a negative regulatory factor of RAS, which can balance multiple functions of ACE. By regulating angiotensin II, ACE2 can cleave angiotensin II into Ang1-7, to protect heart and relax blood vessels. It is also one of the key active receptors in the field of pharmaceutical science research. The principle of this kit is that ACE2 catalyzes the decomposition of substrates, releasing fluorescent products. The higher the fluorescence value, the higher the ACE2 activity in the sample. The ACE2 enzyme activity in the sample can be calculated by the standard curve. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=325 nm/395 nm) |
| Research Area | Metabolic Diseases |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.028 U/L |
| Detection Range | 0.028-10.47 U/L |
| Precision | inter-assay CV: 4.4-11.8% | intra-assay CV: 1.5-3.7% |
| Sample Volume | 10 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse kidney tissue homogenization | 2-8 |
| 10% Mouse lung tissue homogenization | 1-5 |
| 10% Mouse heart tissue homogenization | 1 |
| 10% Mouse liver tissue homogenization | 1 |
| 1 × 10^6 293 cell | 1 |
| 1 × 10^6 HL-60 cell | 1-2 |
| 1 × 10^6 Hela cell | 1-2 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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