γ-Aminobutyric Acid (GABA) Fluorometric Assay Kit (E-BC-F048)
For research use only.
| Detection Principle |
γ-Aminobutyric Acid (GABA) is a major inhibitory neurotransmitter in the mammalian central nervous system. GABA also has the function of promoting mental stability, regulating blood pressure level, improving brain vitality, increasing growth hormone secretion, accelerating ethanol metabolism and providing nutrients to nerve cells. GABA is catalyzed by enzyme, and the removed hydrogen will reduce NADP+ to NADPH, and the GABA content of the sample can be calculated by measuring the generated amount of NADPH. |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 10.74 μmol/L |
| Detection Range | 10.74-200 μmol/L |
| Precision | inter-assay CV: 3.1-9.5% | intra-assay CV: 3.2-4.5% |
| Sample Volume | 20 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Rabbit serum | 15-25 |
| Rat serum | 20-40 |
| Mouse plasma | 15-25 |
| Human serum | 15-25 |
| 10% Mouse liver tissue homogenate | 15-20 |
| 10% Mouse brain tissue homogenate | 30-45 |
| 1×10^6 Molt-4 cells | 1 |
| 1×10^6 RAW cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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