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For research use only.
Detection Principle | Phenol and sodium hypochlorite react with GABA to produce a blue-green product, which has maximum absorbance at 640 nm. GABA content can be calculated with the absorbance at 640 nm. |
Sample Type | Plant,Animal tissue |
Detection Method | Colorimetric method |
Detection Instrument | Microplate reader (630-650 nm, optimum wavelength: 640 nm) |
Research Area | Amino Acids And Proteins, Plant Physiology Series |
Storage | This product can be stored at 2-8°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 0.06 μmol/mL |
Detection Range | 0.06-10.0 μmol/mL |
Precision | inter-assay CV: 6.8% | intra-assay CV: 4% |
Sample Volume | 30 μL(tissue homogenate) |
Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
10% Epipremnum aureum tissue homogenization | 1 |
10% Green pepper tissue homogenization | 1 |
10% Chinese yam tissue homogenization | 1 |
10% Rat heart tissue homogenization | 1 |
10% Rat liver tissue homogenization | 1 |
10% Rat kidney tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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