Aldo-Keto Reductase (ARKs) Activity Assay Kit (E-BC-K810-M)
For research use only.
| Detection Principle | Aldo-keto reductase (AKRS) is distributed in prokaryotes and eukaryotes, and there are a variety of isoforms. AKRS can catalyze the NADPH-dependent carbonyl reduction reaction to generate the corresponding alcohols. Studies have shown that AKRS are associated with a variety of cancers. The principle of this kit is that the substrate is catalyzed by AKRS, and the chromogenic substance produced by the reaction of the product and the chromogenic agent has a characteristic absorption peak at 450 nm. By detecting the amount of chromogenic substance produced per unit time, the activity of AKRS enzyme in the sample can be calculated. |
| Synonyms | ARKs |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Double distilled water, Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.05 U/L |
| Detection Range | 0.05-45.00 U/L |
| Precision | inter-assay CV: 7.1-10% | intra-assay CV: 1.9-4.8% |
| Sample Volume | 40 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse kidney tissue homogenization | 1 |
| 10% Mouse brain tissue homogenization | 1 |
| 10% Mouse heart tissue homogenization | 1 |
| 10% Mouse liver tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| Mouse plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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