ALDH1/ALDH1A1/ALDC Monoclonal Antibody (AN200041P)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2 Verified Samples in FCM: A549 |
| Dilution | WB 1:500-1:2000, FCM 1:25-1:100, ICC/IF 1:20-1:100, |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, FCM, ICC/IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human ALDH1 protein |
| Abbre | ALDH1A1 |
| Synonyms | Retinal Dehydrogenase, Aldehyde Dehydrogenase Family 1 Member A, ALDH1A, ALDH-E, HEL, ALDH1A1, ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1, Aldehyde Dehydrogenase Cytosolic, Aldehyde Dehydrogenase Family 1 Member A1, ALHDII, PUMB10, RALDH 1, Retinal Dehydrogenase 1 |
| Swissprot | |
| Calculated MW | 55 kDa |
| Observed MW |
55 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Stem Cells, Cancer |
| Clone No. | 11A4 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. |
Other Clones
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Unconjugated
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