Aldehyde Dehydrogenase (ALDH) Fluorometric Activity Assay Kit (E-BC-F044)
For research use only.
| Detection Principle | The detection principle of this kit is that the substrate under the action of aldehyde dehydrogenase transforms NAD+ into NADH, NADH reacts with the fluorescent probe to form fluorescent substance under the action of enzymes. The activity of ALDH can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm. |
| Synonyms | ALDH |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 μmol/L |
| Detection Range | 0.06-3 μmol/L |
| Precision | inter-assay CV: 8.4% | intra-assay CV: 0.04 |
| Sample Volume | 40 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 150-160 |
| 10% Mouse kidney tissue homogenate | 90-150 |
| 10% Mouse liver tissue homogenate | 150-180 |
| 10% Mouse lung tissue homogenate | 60-90 |
| 10% Mouse brain tissue homogenate | 30-40 |
| 10% Mouse spleen tissue homogenate | 60-150 |
| Mouse plasma | 3-5 |
| Jurkat cell | 8-12 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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