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AGPAT2 Polyclonal Antibody (E-AB-61187)

AllSizePriceQty
200μL $ 530.00
120μL $ 320.00
60μL $ 200.00
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For research use only.

Verified Samples Verified Samples in WB: HT29
Verified Samples in IF: HeLa
Dilution WB 1:500-1:2000,  IF 1:50-1:100
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse
Applications WB,  IF
ClonalityPolyclonal
ImmunogenRecombinant fusion protein of human AGPAT2 (NP_006403.2).
AbbreAGPAT2
Synonyms1-AGPAT2,  AGPAT2,  BSCL,  BSCL1,  LPAAB,  LPAAT-beta
Swissprot
Calculated MW27 kDa/30 kDa
Observed MW 31 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular LocalizationMembrane.
Concentration1 mg/mL
BufferPhosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification MethodAffinity purification
Research AreasCancer,  Cardiovascular,  Metabolism
ConjugationUnconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
ShippingThe product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
backgroundThis gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Other Clones

1 Results

    Other Formats

    1 Results

    Unconjugated

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