Adenosine Diphosphate Glucose Prophosphorylase(AGP) Activity Assay Kit (E-BC-K856-M)
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For research use only.
| Detection Principle | Adenosine diphosphate glucose pyrophosphorylase (AGP) catalyzes the substrate to produce an intermediate product, which produce chromogenic substances after enzyme catalysis. The substance has a maximum absorption peak at around 450 nm, and the AGP activity can be calculated by the OD value at 450 nm. |
| Synonyms | AGP |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.5 U/L |
| Detection Range | 0.5-81.59 U/L |
| Precision | inter-assay CV: 5.5-10.0% | intra-assay CV: 3.0-4.2% |
| Sample Volume | 40 μL(tissue homogenate) |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Corn tissue homogenate | 1 |
| 10% Epipremnum aureum tissue homogenate | 1 |
| 10% Asparagus tissue homogenate | 1 |
| 10% Barley tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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