Adenosine Colorimetric Assay Kit (E-BC-K833-M)
For research use only.
| Detection Principle |
Adenosine is an endogenous nucleoside distributed throughout human cells. It can directly enter the myocardium and be phosphorylated to produce adenosine, which is involved in myocardial energy metabolism, and also participates in the dilation of coronary vessels and increase blood flow. Adenosine has physiological effects on the cardiovascular system and many other systems and tissues of the body. It also promotes the growth of human hair follicles and the proliferation of dermal papilla cells in vitro. The detection principle of this kit is that the substances produced by the enzyme-catalyzed adenosine reaction consume NADH, the OD value of the system decreases at 340 nm, and the content of adenosine can be calculated by calculating the decline rate at 340 nm. |
| Sample Type | Serum,Plasma,Animal and plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Research Area | Metabolic Diseases , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.027 mmol/L |
| Detection Range | 0.027-1.000 mmol/L |
| Precision | inter-assay CV: 1.9-6.8% | intra-assay CV: 3.5-5.8% |
| Sample Volume | 100-150 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 293T cells | 1 |
| Mouse plasma | 1 |
| Human serum | 1 |
| Rat serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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