5’-Nucleotidase (5'-NT) Activity Assay Kit (E-BC-K196-M)
For research use only.
| Detection Principle | 5'-Nucleotide enzyme (5'-NT, EC 3.1.3.5), full name is 5'-ribonucleotide phosphohydrolase, is a special phosphohydrolase that specifically hydrolyzes 5'-phosphoric acid attached to pentose in 5'-nucleotide. This enzyme is widely distributed in the cell membrane of various tissues of human and animal. Only the 5'-NT released by the tissue cells of the hepatobiliary system may enter the blood. Therefore, the source of serum 5'-NT has certain specificity, and the determination of serum 5'-NT has important value for the diagnosis of hepatobiliary diseases. |
| Synonyms | 5'-NT |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (550 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 28.0 U/L |
| Detection Range | 28.0-581 U/L |
| Precision | inter-assay CV: 0.06 | intra-assay CV: 3.3% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 2-5 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 2-5 |
| Rat plasma | 1 |
| Dog serum | 1 |
| Human serum | 1 |
| Mouse plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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