3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGR) Activity Colorimetric Assay Kit (E-BC-K795-M)
For research use only.
| Detection Principle |
3-Hydroxy-3-methyl glutaric acyl coenzyme A reductase (hydroxy methyl glutaric acyl coenzyme A reductase, HMGR) is a hydroxy valeric acid way to control of the rate of enzyme, A hydroxy valeric acid way is acetyl CoA-produces cholesterol metabolic pathways, HMGR detection kit is cholesterol and other related metabolic pathways important means of basic research. The detection principle of this kit is as follows: HMGR catalyzes the substrate reaction to consume NADPH, causing a decrease in absorbance at 340 nm, and the activity of HMGR can be calculated by measuring its decline rate. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Metabolic Diseases |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.67 U/L |
| Detection Range | 0.67-24.65 U/L |
| Precision | inter-assay CV: 4.2-10.0% | intra-assay CV: 4.2-6.0% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse calf muscle tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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