β-1,3-Glucanase Activity Colorimetric Assay Kit (E-BC-K1223-M)
For research use only.
| Detection Principle |
β-1, 3-glucanase (β-1,3-GA, EC 3.2.1.39) is mainly found in plants and catalyzes
the hydrolysis of β-1, 3-glucosidic bonds, thereby destroying fungal cell walls. In
particular, when synergistic with chitinase, it can significantly inhibit the growth
of fungi. When plants are infected with diseases or under other stress conditions,
they can induce a large amount of β-1, 3-GA to enhance plant resistance to
adverse external stimuli. Therefore, the determination of β-1, 3-GA activity is
widely used in plant pathology and stress physiology research. β-1,3-GA hydrolyses the β-1, 3-glucosidic linkage of laminarin to produce reducing ends. The activity can be reflected by measuring the amount of reducing sugar using 3, 5-dinitrosalicylic acid. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-550 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | 95% alcohol, 80% alcohol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 15.33 U/mL |
| Detection Range | 6.22-333.33 U/mL |
| Precision | inter-assay CV: 2.6-3.8% | intra-assay CV: 4.1-6.4% |
| Sample Volume | 40 μL(tissue homogenate) |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 20% Epipremnum aureum tissue homogenate | 1 |
| 20% Corn kernel tissue homogenate | 1 |
| 20% Sweet potato tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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