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Rat/Chicken CORT (Corticosterone) ELISA Kit (E-EL-0160)

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All Size Price Qty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 0.24 ng/mL
Detection Range 0.39-25 ng/mL
Sample Volume 50 μL
Total Assay Time 2 h 30 min
Reacitivity Rat;Chicken
Specificity This kit recognizes Rat, Chicken CORT in samples.No significant cross-reactivity or interference between Rat, Chicken CORT and analogues was observed
Recovery 80%-120%
Sample Type serum, plasma, urine
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat, Chicken CORT. During the reaction, Rat, Chicken CORT in the sample or standard competes with a fixed amount of Rat, Chicken CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat, Chicken CORT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat, Chicken CORT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Corticosterone is the adrenal steroid, the major glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are synthesized mainly in the adrenal cortex; however, more recently the enzymes involved in their synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated via both cytoplasmic mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs), which act as ligand-inducible transcription factor. Corticosterone has profound effect on the structure and function of the hippocampus. Brain corticosterone action through the glucocorticoid receptor may involve memory storage. Emotional stress might cause increases in plasma corticosterone.
Research Area Signal Transduction , Cancer , Epigenetics And Nuclear Signal , Neuroscience , Cardiovascular , Cell Biology , Stem Cells , Metabolism
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