One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594) (E-CK-A322)
- +5
For research use only. Order now, ship in 3 days
| Detection Principle | When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope. |
| Detection Method | Fluorometric method |
| Sample Type | Paraffin section;frozen section;cell slide |
| Assay Time | 3 hours |
| Detection Instrument | Fluorescence Microscope |
| Dye Type | Elab Fluor®594 |
| Ex/Em | 590/617 |
| Filter Set | TRITC |
| Other Reagents Required | 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Expiration date | 12 months |
| Shipping | Ice bag |
Other Clones
1 Results
Other Formats
1 Results
- PRDM16 deficiency promotes podocyte injury by impairing insulin receptor signaling
IF：13.7
Journal：CELL DEATH AND DIFFERENTIATION(2025)
DOI：10.1038/s41418-025-01477-9 - Ubiquitin-specific protease 25 improves myocardial ischemia–reperfusion injury by deubiquitinating NLRP3 and negatively regulating NLRP3 inflammasome activity in cardiomyocytes
IF：7.9
Journal：Clinical and Translational Medicine(2025)
DOI：10.1002/ctm2.70243 - GSDMD Deficiency Attenuates the Development of Ascending Aortic Dissections in a Novel Mouse Model
IF：7.4
Journal：ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY(2025)
DOI：10.1161/ATVBAHA.124.321740 - Quinolinic acid potentially links kidney injury to brain toxicity
IF：6.3
Journal：JCI Insight(2025)
DOI：10.1172/jci.insight.180229 - Mitochondrial Mayhem: How cigarette smoke induces placental dysfunction through MMS19 degradation
IF：6.2
Journal：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY(2025)
DOI：10.1016/j.ecoenv.2025.117728 - A network toxicology and machine learning approach to investigate the mechanism of kidney injury from melamine and cyanuric acid co-exposure
IF：6.2
Journal：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY(2025)
DOI：10.1016/j.ecoenv.2025.118029 - Magea13 attenuates myocardial injury in acute myocardial infarction by inhibiting the cAMP-PKA signaling pathway
IF：6.1
Journal：APOPTOSIS(2025)
DOI：10.1007/s10495-025-02078-0 - Shuanglu tongnao formula alleviates cerebral ischemia/reperfusion injury by rebuilding inflammatory microenvironment after cerebral ischemia
IF：4.8
Journal：JOURNAL OF ETHNOPHARMACOLOGY(2025)
DOI：10.1016/j.jep.2025.119640 - Vegfr3 activation of Pkd2l1+ CSF-cNs triggers the neural stem cell response in spinal cord injury
IF：4.4
Journal：CELLULAR SIGNALLING(2025)
DOI：10.1016/j.cellsig.2025.111675 - HnRNPM modulates alternative splicing in germ cells by recruiting PTBP1
IF：4.2
Journal：Reproductive Biology and Endocrinology(2025)
DOI：10.1186/s12958-024-01340-5
Q1:What thickness do you recommend for frozen slices?
Generally, the thickness of frozen slices is 8-10 µm. If the slice is thick, the incubation time of protease K can be extended appropriately according to the actual situation.
Q2:What is the well state of dewaxing?
The tissue is transparent, and no water drop can be left on the slide.
Q3:What is the role of the TdT equilibration buffer in the TUNEL Kit?
The TdT equilibration buffer in the TUNEL kit is designed to provide optimal reaction conditions for the TdT enzyme.
Q4:What is the resolution ratio of CCK-8?
The best linearity of CCK-8 is between 0.2-2.5 (Optical Density).
Q5:What is the recommended amount of PBS washing?
PBS added during washing steps must completely cover the sample, and ensure the sample moist during the experiment to prevent the failure of the experiment caused by dry slides.
Q6:What is the purpose of using equilibrition buffer before labeling?
The equilibrition process is to make a better working condition of TdT enzyme that benefit a better staining result.
Q7:What is the difference between TUNEL-fluorescence and HRP-DAB TUNEL?
Color development of DAB (result of TUNEL-HRP) is observed with an ordinary microscope, the results of TUNEL nuclear staining were served with white light. As for TUNEL fluorescence, the results of TUNEL and nuclear staining were observed with different fluorescence channels seperately with better definition and resolution.
Q8:What is the difference between TUNEL in-situ kit and TUNEL flow cytometry kit?
The main difference lies in the detection methods, for TUNEL in-situ kits, a fluorescence microscope is used for observing the results, as for TUNEL flow ytometry kit, the sample will be detected by a flow cytometer.
On the other hand, TUNEL in-situ kit is more suitable for tissue sections and cell slides or smears, while TUNEL flow ytometry kit is more suitable for suspended cells and adherent cells.Q9:What is the difference between one-step and two-step Annexin V operation?
Both systems have the same resolution ratio, while one-step operation takes easier process, and two-step operation requires less reagent.
Q10:What are the reasons that lead to false positives in TUNEL?
Cells with high proliferative or metabolic active may have a break in the DNA strand, both cases may end up with false-positive result. Evaluation of cell morphology may be used if there are doubts about the interpretation of the results.
